Chlorophyll

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Chlorophyll Determination


Hettich centrifuges have applications in environmental analysis. You can centrifuge water samples to determine chlorophyll a or phaeophytin content. The concentration of chlorophyll permits a statement on water quality.


Procedure*

The plant mass present in a water sample is first enriched through filtration. It is then homogenised and extracted. The substance under test enters the solvent (e.g. acetone). The extract thus derived is therefore clarified prior to analysis.


Laboratory centrifuges are used for this clarification process. They have the advantage over filtration that there is no waste and less labour is required. For large numbers of samples the space requirement is lower and a higher number of samples can be processed in the time available.


Centrifugation Parameters
  • Speed (RPM): 3,500 min-1 to 4,500 min-1
  • Running time: 10 to 20 minutes
  • Volume: 10 to 30 ml
  • Temperature: 10 °C to 15 °C

Procedure used in the Chlorophyll a ring study.
Final Report of the Umweltbundesamt [Federal Environment Agency] / FG II 3.3 and II 3.5, November 2002. Berlin.


Ordering Information
Centrifuge Cat. No.
UNIVERSAL 320R 110-127V 60Hz 1406-01

Selection of accessories Cat. No.
Rotor, swing-out,4 place 1624
Bucket, round 1430
Lid, bio-containment for 1381, 1430, 1390 1382
Adapter 30ml skirted (1 each) 1365