Hettich EBA 20 Tabletop Centrifuge Featured in High Fructose Diet Study
The Pakistan Journal of Nutrition published a study entitled “Evaluating of High Fructose Diet to Induce Hyperglycemia and its Inflammatory Complications in Rats” that aimed to assist in elevating the effectiveness of dietary compounds to prevent certain diseases, specifically Diabetes Mellitus (DM). The Hettich EBA20 tabletop centrifuge was used in the procedure for blood glucose measurement.
The study examined the effect of high fructose-level administration on blood glucose, C-reactive protein, interleukin-6 (IL-6), interleukin-4 (IL-4), and body weight among fructose diet-induced male Sprague-Dawleyrats. The high fructose diet was delivered to the rats through drinking water in 21% concentration for 10 weeks. The rats were fasted overnight before blood samples were taken, via tail incision, to measure blood glucose levels. Inflammatory biomarkers were identified by anesthetizing the rats withslight diethyl ether exposure. Blood was collected after and centrifuged with the Hettich EBA 20 at 3000rpm for 10 minutes. C-reactive protein, IL-6 and IL-4 plasma levels were quantified at the beginning and end of the 10-week high fructose diet induction.
Other methods utilized to complete the study included the dissection and examination of the rats’ organs. Analysis of the data and results showed that the high fructose diet administration was unable to induce hyperglycemia, hypertriglyceridemia or other classic inflammatory biomarkers. Meanwhile, non-genetic environmental factors such as diet were able to prevent or induce the onset of numerous diseases.
Hettich’s comprehensive line of centrifuges enables scientists to conduct important testing and research, such as this high fructose diet study in male Sprague-Dawley rats. The Hettich EBA 20 is a compact, quiet, safe and reliable centrifuge that is suitable for clinical testing and research in many fields. Learn more about the Hettich EBA 20 centrifuge at http://www.hettweb.com/hettich-eba-20-centrifuge.html.