Hettich MIKRO 120 Microliter Centrifuge Used to Obtain High Purity Antibodies from Hen Egg Yolks
A team of Romanian scientists used the Hettich MIKRO 120 microliter centrifuge when extracting high purity yolk immunoglobulins (IgY) from hen eggs in a study entitled “Obtaining High Purity Antibodies With Therapeutic Potential.” The study aimed to establish an inexpensive, optimized method for generating high purity antibodies from egg yolk that can be used for pharmaceutical and therapeutic purposes.
Immunoglobulinsare transmitted from hens’ blood into the egg yolk. Studies show that IgY can contain antibodies against up to 200 antigens, making it ideal for wide-ranging immunological and diagnostic applications, such as the prophylaxis and therapy of infectious diseases. However, because of the high lipid content of egg yolk, IgY extraction is difficult.
As an alternative to extracting IgY from actual egg yolk, in the study researchers developed a method in which they extracted IgY from the product Globigen® Pig Doser, “an oil suspension of dried egg powder obtained from hens immunized with specific antigens.” They then compared their new method with three classical methods, testing for both IgY purity and total protein yield.
The Hettich MIKRO 120 microliter centrifuge was employed in the classical poloyethylene glycol (PEG) method and the chloroform-PEG method executedto test against the new method. In each procedure, a mixture containing egg yolk was centrifuged at 14,000 x g and the resulting supernatant was precipitated to achieve IgY.
The authors concluded that the extraction of IgY from the product Globigen® Pig Doserproved to be “a convenient, fast, and readily available method for obtaining electrophoretically pure antibodies.”
Hettich’s comprehensive line of centrifuges enables scientists to conduct important testing and research, such as this innovative study on IgY extraction methods.The Hettich MIKRO 120 is a powerful, ultra-compact microliter centrifuge that is safe, quiet and reliable.
Learn more about the Hettich Mikro 120 centrifuge packages at http://www.hettweb.com/hettich-mikro-120-centrifuge.html.