Hettich ROTOFIX 32 Used For Defining Normal Haematological and Biochemical Parameters in Rabbits
The World Rabbit Science journal published a study entitled, “Normal Values of Haematological and Some Biochemical Parameters in Serum and Urine of New Zealand White Rabbits”, conducted by Cumali Özkan, Abdullah Kaya, and Yakup Akgül. The researchers employed the Hettich ROTOFIX 32 centrifuge in their experiment to “define normal haematological values and some biological parameters in serum and in urine in both male and female New Zealand white rabbits and to determine the effect of gender on these parameters.”
According to the study, the New Zealand white rabbit is commonly used in laboratory studies for its “high fertility, easier availability, feeding and housing features.” The study notes that because “disorders have considerable effects on blood parameters in rabbits, it is important to analyze [their] blood and urine.” However, “the lack of reference values for biochemical parameters in rabbits leads to problems for many clinicians and constrains many researchers.”
To gain reference values for the rabbits, blood and urine samples from 40 male and female New Zealand white rabbits were collected and analyzed. The blood samples were used to determine haematologic parameters, while the serum and urine samples were used to determine biochemical parameters.
The Hettich ROTOFIX 32 was used to centrifuge the blood samples collected into test tubes without anticoagulant at 3000 rpm for 10 minutes. The urine samples were also centrifuged at 3,000 rpm for 10 minutes to obtain the supernatant used to measure urine protein, glucose, creatinine, urea, γ-glutamyl transferase (GGT), nitrite, Na, K and Cl levels.
Normal values were then determined for each parameter and statistical comparisons were conducted between the male and female rabbits. Results of the study showed no significant difference between the male and female animals for the parameters analyzed with the exception of HCT, HGB, granulocyte %, L/M and serum K concentration.
Hettich provides a wide range of benchtop, tabletop, and floor-standing centrifuges for clinical, research, and industrial applications. The ROTOFIX 32 is a rugged general-purpose benchtop centrifuge ideal for routine laboratory tasks. To learn more about the Hettich ROTOFIX 32 centrifuge, visit http://www.hettweb.com/hettich-rotofix-32a-centrifuge.html.