Hettich UNIVERSAL 320 Benchtop Centrifuge Featured in Cell-Specific Analysis of IAA Distribution and Synthesis within Arabidopsis Root Apex
The Plant Cell volume XXI featured a study analyzing indole-3-acetic acid (IAA) distribution and synthesis within the plant Arabidopsis’ (Arabidopsis thaliana) root apex. Researchers used the Hettich UNIVERSAL 320 benchtop centrifuge for protoplast isolation.
Arabidopsis thaliana, or simply Arabidopsis, is a small flowering plant native to Europe and Asia. It is used to study the molecular biology of many plant traits, such as plant development and light sensitivity.
IAA synthesis rates within Arabidopsis’ root apex were obtained using various methods and techniques, including centrifugation. The study states that both pectolyase and cellulysin were dissolved in a protoplast isolation buffer to a specific final concentration. The apical part of the plant’s root, which measured approximately one-third of its total length, was collected. It was then incubated in the resulting buffer for 2 hours at 22 °C with gentle stirring at 110 rpm. The remaining tissue was filtered, and the protoplasts were centrifuged at 1000 RCF with the Hettich UNIVERSAL 320.
A protoplast is a plant cell with a partially or completely removed cell wall, and its isolation was crucial for accurate analysis of IAA synthesis and distribution in the plant’s root apex. IAA acid is the most common auxin plant hormone. It is crucial for normal plant development, including plants’ responses to environmental factors like gravity and light. Fluorescence-activated cell sorting and mass spectrometry were also used to directly quantify IAA and biosynthesis measurements.
Hettich’s comprehensive line of centrifuges enables scientists to conduct important testing and research, such as this study published by The Plant Cell. The Hettich UNIVERSAL 320 is a multifunctional, quiet, safe and reliable centrifuge that is suited for many applications in clinical, research, and industrial laboratories. Learn more about the Hettich UNIVERSAL 320 at http://www.hettweb.com/Products/hettich-universal-320-centrifuge.html.