Chlorophyll Determination

Hettich centrifuges in environmental analysis Determination of chlorophyll content as an indicator of water purity

You can centrifuge water samples to determine chlorophyll and phaeophytin content. The concentration of chlorophyll permits a statement on water quality.

PROCEDURE*

The plant mass present in a water sample is first enriched through filtration. It is then homogenized and extracted. The substance under test enters the solvent (e.g. acetone). The extract thus derived is therefore clarified prior to analysis.

Laboratory centrifuges are used for this clarification process. They have the advantage over filtration that there is no waste and less labor is required. For large numbers of samples, the space requirement is lower and a higher number of samples can be processed in the time available.

CENTRIFUGE PARAMETERS

Speed (RPM): 3,500 min-1 to 4,500 min-1
Running time: 10 to 20 minutes
Volume: 10 to 30 ml
Temperature: 10 °C to 15 °C

ORDERING INFORMATION