Chlorophyll Determination

Centrifuge Application

Hettich Centrifuge Application

Hettich centrifuges in environmental analysis Determination of chlorophyll content as an indicator of water purity

You can centrifuge water samples to determine chlorophyll and phaeophytin content. The concentration of chlorophyll permits a statement on water quality.


The plant mass present in a water sample is first enriched through filtration. It is then homogenized and extracted. The substance under test enters the solvent (e.g. acetone). The extract thus derived is therefore clarified prior to analysis.

Laboratory centrifuges are used for this clarification process. They have the advantage over filtration that there is no waste and less labor is required. For large numbers of samples, the space requirement is lower and a higher number of samples can be processed in the time available.


Speed (RPM): 3,500 min-1 to 4,500 min-1
Running time: 10 to 20 minutes
Volume: 10 to 30 ml
Temperature: 10 °C to 15 °C

*Procedure used in the Chlorophyll a ring study. Final Report of the Umweltbundesamt [Federal Environment Agency] / FG II 3.3 and II 3.5, November 2002. Berlin.


Centrifuge Cat. No.
UNIVERSAL 320R 1406-01


Selection of accessories Cat. No.
Rotor, swing-out,4 place 1624
Bucket, round 1430
Lid, bio-containment for 1381, 1430, 1390 1382
Adapter 30ml skirted (1 each) 1365


Hettich products are designed to help you achieve optimal results for your application and are built to perform to the specifications outlined in the operators manual. For application-specific information and settings, please refer to your organization’s standard operating procedure. As always, our Hettich representatives are here to help determine which Hettich products and accessories best fit your laboratory requirements.


Chlorophyll Determination Application Note

This application note provides information on specific application methods and the use of Hettich products. (more…) read more...

File type: PDF
File size: 286.1 kb
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Hettich UNIVERSAL 320 R benchtop centrifuge product sheet

UNIVERSAL 320 | R Product Sheet

This product sheets provides a comprehensive overview of the unique product features specific to the model. (more…) read more...

File type: PDF
File size: 118.2 kb
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Hettich manufactures centrifuges for any standard laboratory application.