Hettich centrifuges in environmental analysis Determination of chlorophyll content as an indicator of water purity
You can centrifuge water samples to determine chlorophyll and phaeophytin content. The concentration of chlorophyll permits a statement on water quality.
PROCEDURE*
The plant mass present in a water sample is first enriched through filtration. It is then homogenized and extracted. The substance under test enters the solvent (e.g. acetone). The extract thus derived is therefore clarified prior to analysis.
Laboratory centrifuges are used for this clarification process. They have the advantage over filtration that there is no waste and less labor is required. For large numbers of samples, the space requirement is lower and a higher number of samples can be processed in the time available.
CENTRIFUGE PARAMETERS
Speed (RPM): 3,500 min-1 to 4,500 min-1
Running time: 10 to 20 minutes
Volume: 10 to 30 ml
Temperature: 10 °C to 15 °C
*Procedure used in the Chlorophyll a ring study. Final Report of the Umweltbundesamt [Federal Environment Agency] / FG II 3.3 and II 3.5, November 2002. Berlin.
ORDERING INFORMATION
| Centrifuge | Cat. No. |
|---|---|
| UNIVERSAL 320R | 1406-01 |
| Selection of accessories | Cat. No. |
|---|---|
| Rotor, swing-out,4 place | 1624 |
| Bucket, round | 1430 |
| Lid, bio-containment for 1381, 1430, 1390 | 1382 |
| Adapter 30ml skirted (1 each) | 1365 |
