Coliform Bacteria in Dairy Products

Incubator Application

Hettich Incubator Application

The use of HettCube incubators and cooled incubators to detect the presence of coliform bacteria in milk and dairy products

Coliform bacteria are facultative pathogens and can cause systemic infections in people whose immune systems are weakened. They are also crucial in quality control and as indicators of the sanitary quality of facilities and food products.


Coliform bacteria can be demonstrated in either liquid or solid media per din 10 172-1 and 172-3. A liquid selective medium is inoculated with the diluted sample and is incubated in a test tube containing a durham tube. The evolution of gas inside the durham tube indicates the presence of coliform bacteria. As an alternative, the diluted sample can be mixed in a Petri dish with a selective medium that is liquefied and cooled to 47 °C. As soon as the mixture has solidified, it is incubated. Coliform bacteria appear as typical dark red colonies.

Importance of the demonstration of coliform bacteria in milk and dairy products

Milk and dairy products are tested for the presence of coliform bacteria at regular intervals by manufacturers and the regulatory authorities to verify that the procedures adopted during production processes and distribution meet hygiene requirements.

Hettich products are designed to help you achieve optimal results for your application and are built to perform to the specifications outlined in the operators manual. For application-specific information and settings, please refer to your organization’s standard operating procedure. As always, our Hettich representatives are here to help determine which Hettich products and accessories best fit your laboratory requirements.

HettCube Incubators

Hettich Hettcube incubators and cooled incubators are built to maximize their compact footprint with efficient design features that improve temperature uniformity, operational performance, and user interaction.

HettCube series of incubators


Coliform Bacteria in Dairy Products Application Note

This application note provides information on specific application methods and the use of Hettich products. (more…) read more...

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Hettich HettCube incubators product sheet

HettCube Product Sheet

This product sheets provides a comprehensive overview of the unique product features specific to the model. (more…) read more...

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