Posted Mar 02

PCR

In Molecular Biology, PCR (polymerase chain reaction) is used to amplify pieces of DNA through a number of cycles into millions of copies of the DNA sequence. The technique relies on Thermal Cycling, which consists of a heating and cooling sequence to separate the DNA double helix into single strands for amplification...

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Posted Mar 02

Chlorophyll Determination

Hettich centrifuges have applications in environmental analysis. You can centrifuge water samples to determine chlorophyll a or phaeophytin content. The concentration of chlorophyll permits a statement on water quality. Procedure* The plant mass present in a water sample is first enriched through filtration. It is then homogenised and extracted. The substance under test...

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Posted Mar 02

Sulfate Concentration

Determination of Sulfate Concentration Hettich centrifuges can be used to determine the sulfate concentration in chrome baths. To ensure a high quality result when using chrome baths, their sulfuric acid concentration must be maintained at an optimum level. This concentration will change over time with use of a chrome bath. As a preventive...

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Posted Dec 23

Urine

To derive a good cytological preparation from a urine sample it is not sufficient to simply centrifuge native urine onto commercial microscope slides. Urine has a low cell concentration and those cells present adhere poorly to the slides. This would result in a preparation with an insufficient cell concentration. To increase the...

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Posted Dec 23

Water Retention Value in Pulp and Paper

Determination of the Water Retention Value (WRV) in Pulp and Paper Manufacturing The water retention value (WRV) is an important parameter in the manufacture of pulp and paper. It is an indication of the ability of a sample of wood fibre or pulp mass to retain water. The WRV changes in the...

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Posted Dec 23

Pleural or Ascitic Fluid

In addition to mesothelial cells, pleural fluid and ascitic fluid also contain lymphocytes and eosinophilic and neutrophilic granulocytes. The protein content is frequently around 30 g/l. The cell content often varies considerably. Both fluids may contain blood and ascitic fluid may also contain lipids. Advantages of the Hettich Method Cyto chambers for...

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