Hettich ROTANTA 460R Used In Directed Neuronal Differentiation Study
The study entitled “Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells” discusses the endogenous differentiation potential of neurosphere cells derived from both embryonic and adult brain tissues. The paper provides protocols on how to direct adult subependymal zone (SEZ) neurosphere cells toward specific neuronal subtypes, such as GABAergic, through retroviral-mediated gene transfer of proneural genes.
According to the research, neurosphere culture can propagate cells derived from several neural tissues with the potential to self-renew and generate neurons, astrocytes, and oligodendrocytes, in vitro. These neurosphere-forming cells can be isolated from the embryonic brain and continue to exist in some brain regions throughout adulthood. The cells were also noted for being a potential source of newly generated cells for brain repair.
The cell suspensions used in the differentiation of dissociated cells were centrifuged at 1,000 rpm for 5 minutes using the Hettich ROTANTA 460R. By selectively differentiating adult neurosphere cells, the study opens up the possibility for these cells to be directed toward distinct neuronal sublineages. The study also suggests that tailoring adult SEZ-derived neurosphere cells into specific types of degenerating neurons in diseases like amyotrophic lateral sclerosis is feasible.
Hettich offers a comprehensive line of tabletop, benchtop and floor-standing centrifuges for clinical, research and industrial applications. The ROTANTA 460R is a high-capacity, high-throughput benchtop centrifuge supporting an extensive range of accessories. Learn more about the Hettich ROTANTA 460R centrifuge by visiting http://www.hettweb.com/rotanta-460rc-rotanta-460rf.html.